The table below gives details of the technology, sensitivity and detection chemistries of each Golden Bridge International (GBI) detection system. Also provided in the recommended use column are details of when each system/kit may be most useful.
Starting with the recommended use section and looking at the sensitivity you require should provide you with the best indication of which kit to use.
After this consult the Chromogen column and then select the relevant kit name.
Returning to the previous page will then provide you with links to more information on the specific system/kits in question.
This information is provided to help you make an informed choice of which kit may be best for your requirements, however if you are still unsure or have further questions please do not hesitate to use our contact form or email tech@nktscientific.com and we'll be happy to discuss your specific requirements with you...
... after all we want you to get the best results from whatever kit you use!
|
Detection System |
Technology |
Detection Sensitivity |
Chromogen |
Recommended Use |
|
Polink-1 HRP system:
|
One step polymer-HRP detection system (1st generation of polymer technology, biotin free) |
+ + |
DAB or AEC |
Offers quicker turn around with less steps.
Choose Polink-1 HRP DAB kit for DAB staining. Choose Polink-1 HRP AEC kit for AEC staining.
Avoids endogenous biotin non-specific background. |
|
One step polymer-AP detection system (1st generation of polymer technology, biotin-free) |
+ + |
Fast Red AP-Red BCIP/NBT |
Offers quicker turn around with less steps.
Avoids endogenous biotin non-specific background. |
|
|
Polink DS double stain system:
|
One step Polymer-HRP and Polymer-AP detection system (Biotin-free) for review 2 antigens |
+ + |
1. BCIP/NBT & AEC 2. DAB & Fast Red 3. DAB & AP-Red+ |
Provides two polymerized secondaryenzyme conjugates to detect two antigens simultaneously or sequentially.
Available for mouse and rabbit, or two mouse or two rabbit antibodies |
|
Polink-2 HRP system:
|
Two step polymer-HRP detection system (2nd generation of polymer technology, biotin-free). More sensitive than one step polymer system. |
+ + + + |
DAB or AEC |
Offers higher sensitivity than the Polink-1 system for difficult antibodies.
Avoids endogenous biotin non-specific background.
Only available as a broad spectrum kit.
|
|
|
Two-step polymer-AP detection system (2nd generation of polymer technology, biotin-free). More sensitive than one step polymer system. |
+ + + + |
1. Fast Red 2. AP-Red 3. BCIP/NBT |
Offers higher sensitivity than the Polink-1 system for difficult antibodies.
Avoids endogenous biotin non-specific background.
Only available as a broad spectrum kit. |
|
Polink-2 Plus HRP system:
|
Ultra sensitive two-step polymer-HRP detection system (3rd generation of polymer technology, biotin-free). |
+ + + + |
DAB or AEC
|
Ultra sensitive with low background when potential background issues may be present.
Avoids endogenous biotin non-specific background.
For DAB or AEC staining.
Broad spectrum and single species kits available. |
|
Ultra sensitive two-step polymer-AP detection system (3rd generation of polymer technology, biotin-free). |
+ + + + + |
1. Fast Red 2. AP-Red 3. BCIP/NBT |
Ultra sensitive with low background when potential background issues may be present.
Avoids endogenous biotin non-specific background.
Broad spectrum and single species kits available. |
|
|
HRP Labeled Streptavidin and biotin system |
+ + + + + |
DAB or AEC
|
For general IHC staining with biotin blocking
Lower cost |
|
|
|
Alkaline Phosphatase Labeled Streptavidin-Biotin system |
+ + + |
1. Fast Red 2. AP-Red 3. BCIP/NBT |
For general IHC staining with biotin blocking
Lower cost |
|
Polymer detection systems for animal tissue
|
One-step and two-step polymer-HRP and AP |
+ + + |
1. DAB or AEC 2. Fast Red 3. AP-Red 4. BCIP/NBT |
Follow this link for more information on the kits available. |
