Small RNA Cloning & Delivery
miRNA cloning and library construction, siRNA delivery, RNA isolation
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Our small RNA cloning and delivery range from Bioo Scientific is a rapidly expanding set of tools that facilitates working with small RNAs. Follow the links below for further information and pricing.
Plus read Bioo Scientific's application note "Improved Small RNA Sequencing with Multiplex Compatibility using the Illumina Platform" which was recently published on Nature Methods.
miRNA sequencing, cloning and library construction tools
RNAi delivery reagents
RNAi accessory reagents
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The MiraMas™ Kit from Bioo Scientific uses a novel approach for SYBR Green/qPCR-based profiling of microRNAs and other small RNAs.
The microRNA targets are amplified using microRNA-specific Forward primers and the Universal Reverse primer included in the kit. The Forward primers are designed using a very simple algorithm that does not require modified nucleotides, ensuring the cost for the microRNA-specific primers is minimal. Plus we can even design them for you if you prefer.
Using a convenient one tube protocol we offer researchers great flexibility in terms of sample size and number of reactions performed. The MiraMas Kit overcomes the limitations of other kits for qPCR analysis of microRNA as many more samples can be processed per kit and large panels of miRNA targets can be analysed from each reverse transcription reaction.
Another distinctive feature of the MiraMas Kit is that it includes control RNAs from liver and brain, as well as control Forward primers targeting tissue-specific and constitutively expressed microRNAs. Click here for more information on the MiraMas™ Kit
T3™ Conjugation Kits, based on Bioo Scientific's Targeted Transport Technology, allow you to target the delivery of siRNA into any cell type (in vivo or in vitro) by conjugating the T3 carrier to an antibody that is specific to a receptor or other extracellular domain specific to the cell or tissue you want to target.
The T3 procedure does not require prior modification of the antibody or the RNAi agent and can be performed in a few simple steps. Click here for more information on T3™ Conjugation Kits
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The NEXTflex™ Small RNA Sequencing Kits provide an easy, flexible, cost-effective solution for generating single and multiplexed libraries from small RNA using Illumina GAIIx and HiSeq 2000 sequencing platforms. This kit contains a specialized 3’ adenylated adapter that specifically ligates onto microRNAs and other small RNAs containing a 3’ hydroxyl group. After adapter ligation and reverse transcription, libraries are amplified using a barcoded primer, allowing up to 48 samples to be multiplexed and sequence variations interrogated.
The NEXTflex™ Small RNA Sequencing Kits incorporate AIR™ Ligase, an enhanced, truncated T4 RNA Ligase, which increases the efficiency with which small RNAs are tagged with adapter, giving greater sequence depth.
A NEXTflex™ Small RNA Sequencing Kit on its own contains all the material to prepare 24 or 48 singleplex libraries. In combination with the NEXTflex™ Small RNA Barcode Primer kits, upto 48 samples can be profiled and studied simultaneously. In addition to small RNA sequencing, the kit is compatible with RIP-Seq and CLIP-Seq protocols.
Further information including pricing is available here or alternatively complete our contact form.
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T3™ Conjugation Kits, based on Bioo Scientific's Targeted Transport Technology, allow you to target the delivery of siRNA into any cell type (in vivo or in vitro) by conjugating the T3 carrier to an antibody that is specific to a receptor or other extracellular domain specific to the cell or tissue you want to target.
The T3 procedure does not require prior modification of the antibody or the RNAi agent and can be performed in a few simple steps. Click here for more information on T3™ Conjugation Kits
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MaxSuppressor™ In Vivo RNA-LANCEr II is a proprietary formulation composed of neutral lipid, non-ionic detergent, oil and small molecules which enable highly efficient delivery of RNAi agents into animals. This novel product has been shown to be more robust and efficient at penetrating tissues and organs than other delivery agents.
The procedure to package RNAi agents into the phospholipid-oil emulsion takes less than 1-hour. To use MaxSuppressor™ In Vivo RNA-LANCEr II simply mix 100 ug of your RNAi agent with a single tube of semi-dry MaxSuppressor™ In Vivo RNA-LANCEr II agent in total volume of less than 300 uL, in 1X PBS; extrude using the Lipid Extruder (Cat no 341007) with the supplied 100 nm pore sizing filters (or sonicate) and then directly inject into the animal.
For more information and pricing click here.
Relative GAPDH protein levels in the organs of rats injected with MaxSuppressor™ In Vivo RNA-LANCEr II containing GAPDH siRNA. The relative levels of GAPDH are compared to rats treated with a negative control siRNA.
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