Small RNA Cloning & Delivery

Our small RNA cloning and delivery range from Bioo Scientific is a rapidly expanding set of tools that facilitates working with small RNAs. Follow the links below for further information and pricing.

miRNA sequencing, cloning and library construction tools

• Illumina compatible small RNA sequencing kit
• RNAi Isolation Kit (includes miRNA enrichmnent protocol)
• Adenylated linkers
• Truncated T4 RNA ligase 2 (T4 RNL2)
• Enriched miRNAs from mouse tissues
• ExomiR - RNA extraction from exosomes
• ImpA
• Antibodies to plant miRNAs

RNAi delivery reagents

• Lancefection delivery agent for cell culture
• siRNA delivery agent for hard to transfect cell lines
• MaxSuppressor™ In Vivo RNA-LANCEr II - In-Vivo RNAi delivery agent
• Conjugated Antibodies for the directed delivery of RNAi

RNAi accessory reagents

• Rnase-Free sterile saline and ringers solution
• Anti-siRNA polyclonal antibody
• RNA isolation kit

T3™ Conjugation Kits, based on Bioo Scientific's Targeted Transport Technology, allow you to target the delivery of siRNA into any cell type (in vivo or in vitro) by conjugating the T3 carrier to an antibody that is specific to a receptor or other extracellular domain specific to the cell or tissue you want to target. 

• Offers targeted in vivo or in vitro delivery of siRNA or miRNA
• Can be used to target almost any cell or tissue type
• Flexible - works with multiple RNAi agents and routes of delivery
• Use less siRNA
• Reduces off target effects
• Gentle and non-toxic

The T3 procedure does not require prior modification of the antibody or the RNAi agent and can be performed in a few simple steps. Click here for more information on T3™ Conjugation Kits

The MaxRecovery™ BiooPure™ RNA Isolation Reagent is a single-phase reagent for extraction of total RNA or enriched small RNA (including miRNA) from solid tissues, cultured cells, and cell-free fluids such as serum and plasma.

The BiooPure reagent contains guanidinium, a powerful chaotropic agent effective for rapidly inactivating nucleases, and phenol, an organic solvent used to denature and separate proteins and DNA from RNA. Click here for more information on MaxRecovery™ BiooPure™ RNA Isolation.

AIR Linkers are 5'adenylated linker oligos that facilitate miRNA cloning and sequencing.

Newmarket Scientific now brings you pre-designed AIR™ Adenylated Linkers from Bioo Scientific, available with Ban-1, Ava-1, Sty-1 or EcoR-1 restriction sites. These adenylated linkers are modified to prevent self ligation and improve miRNA cloning efficiency.

Plus Bioo Scientific is the only company that offers custom adenylated linkers which can be designed to meet your precise needs. Contact us for more details.

Features include:

• 3' end blocks self-ligation
• Compatible with all miRNAs
• Can be use for miRNA sequencing
• Custom adenylation services available

Follow this link for more information.

Lancefection™ siRNA Delivery Agent for Cell Culture is a proprietary formulation developed for high efficiency transfection of RNA into eukaryotic cells.

• Compatibile with a broad range of cell types
• Simple, rapid protocol with fewer steps for consistent, reproducible results
• Easy to optimize due to excellent cell viability
• Stable in serum and no need to remove complexes from the cells
• Reverse or forward transfection method
• Efficient at low RNA conc. (<10 nM) with minimal non-specific effects
• Superior adaptability to automated or robotic systems

For further information and pricing for the Lancefection™ siRNA Delivery Agent follow this link.

MaxSuppressor™ In Vivo RNA-LANCEr II is a proprietary formulation composed of neutral lipid, non-ionic detergent, oil and small molecules which enable highly efficient delivery of RNAi agents into animals. This novel product has been shown to be more robust and efficient at penetrating tissues and organs than other delivery agents.

The procedure to package RNAi agents into the phospholipid-oil emulsion takes less than 1-hour. To use MaxSuppressor™ In Vivo RNA-LANCEr II simply mix 100 ug of your RNAi agent with a single tube of semi-dry MaxSuppressor™ In Vivo RNA-LANCEr II agent in total volume of less than 300 uL, in 1X PBS; extrude using the Lipid Extruder (Cat no 341007) with the supplied 100 nm pore sizing filters (or sonicate) and then directly inject into the animal.

For more information and pricing click here.

Relative GAPDH protein levels in the organs of rats injected with MaxSuppressor™ In Vivo RNA-LANCEr II containing GAPDH siRNA.  The relative levels of GAPDH are compared to rats treated with a negative control siRNA.