Next Generation Sequencing
Illumina compatible sample prep kits and reagents
|
Our Illumina compatible NEXTflex™ Sequencing Kits and NEXTflex™ Barcodes from Bioo Scientific have been designed to provide flexibility and high-throughput capabilities in sequencing applications, whilst at the same time providing a significant cost saving. Plus Unique to the Bioo Scientific range:
NEXTflex™ DNA Sequencing Kits NEXTflex™ Small RNA Sequencing Kit NEXTflex™ PCR-Free DNA Sequencing Kits
|
|
|
|
The NEXTflex™ Sequencing Kits can be used to prepare single, paired-end and multiplexed genomic DNA libraries or single and multiplexed small RNA libraries for sequencing using Illumina®/Solexa® GAII and HiSeq 2000/1000 platforms.
The NEXTflex™ DNA Sequencing Kits feature “Enhanced Adapter Ligation Technology” resulting in library preps with a larger number of unique sequencing reads. These enhanced NEXTflex Sequencing Kits simplify workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating several time consuming steps in library preparation. Also changes to the ligation enzymatic mix means the user is now able to perform ligations with longer adapters and can expect to see better binding efficiencies.
This new automation-friendly format enables multiplexing of up to 96 samples for a total of 384 reactions using the NEXTflex™ DNA Barcodes. These adapter containing indexed sequences in combination with the NEXTflex™ DNA Barcode Kits, can be used to profile and study up to 96 samples simultaneously making this the most high-throughput kit available.
The NEXTflex™ DNA Barcodes are available in sets of 6, 12, 24, 48 and 96 unique adapters. The ability to pool samples in an efficient way significantly decreases hands on time while providing robust data quality.
Further information including pricing is available here or alternatively complete our contact form.
The NEXTflex™ Small RNA Sequencing Kits provide an easy, flexible, cost-effective solution for generating single and multiplexed libraries from small RNA using Illumina GAIIx and HiSeq 2000 sequencing platforms. This kit contains a specialized 3’ adenylated adapter that specifically ligates onto microRNAs and other small RNAs containing a 3’ hydroxyl group. After adapter ligation and reverse transcription, libraries are amplified using a barcoded primer, allowing up to 48 samples to be multiplexed and sequence variations interrogated.
The NEXTflex™ Small RNA Sequencing Kits incorporate AIR™ Ligase, an enhanced, truncated T4 RNA Ligase, which increases the efficiency with which small RNAs are tagged with adapter, giving greater sequence depth.
A NEXTflex™ Small RNA Sequencing Kit on its own contains all the material to prepare 24 or 48 singleplex libraries. In combination with the NEXTflex™ Small RNA Barcode Primer kits, upto 48 samples can be profiled and studied simultaneously. In addition to small RNA sequencing, the kit is compatible with RIP-Seq and CLIP-Seq protocols.
Further information including pricing is available here or alternatively complete our contact form.
|
||
|
The AIR™ Small RNA Sequencing Kit has been specifically designed for the preparation of small RNA libraries for sequencing on Illumina/Solexa platforms. Bioo Scientific’s AIR™ Small RNA Sequencing Kit streamlines the sample prep procedure required for the sequencing of microRNAs, piwi RNAs and other non-coding RNAs.
This kit contains all the necessary materials needed to prepare RNA samples for loading onto sequencing flow cells for cluster generation including adapter ligation, first strand synthesis, PCR amplification, band separation and elution.
Click here for pricing and further information _______________________________
Optional Predesigned AIR™ Barcoded Adapters
AIR™ Barcoded Adapters can also be used with the small RNA sequencing kit to provide flexibility in high-throughput sequencing applications. They allow the user to pool multiple library preparations into a single sequencing reaction thereby significantly increasing throughput while reducing costs.
Click here for pricing and further information
For further information please use our contact form or email tech@nktscientific.com.
|
