Hycult Dx

The Hycult Dx Complement factor H ELISA can be used for the in vitro quantitative determination of complement factor H in serum, plasma and urine samples and in combination with other laboratory and clinical findings can provide information about a pathological state.

The human MBL ELISA kit from Hycult Biotech can be used for the in vitro quantitative determination of human MBL in serum and plasma samples.

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Mannan Binding Lectin (MBL), also called mannose binding lectin or mannose/mannan-binding protein (MBP), is a key serum protein involved in innate immunity, and is specifically involved in providing protection from a number of infectious organisms.

The Mannan Binding Lectin (MBL) pathway has been shown to be a major regulator of the complement system with the MBL/MASP complex able to directly activate C3 and is a key player in translating endogenous and exogenous danger into cellular responses. In addition the .

This indicates that the MBL ELISA may be used in combination with other laboratory and clinical findings for the purpose of providing information about a pathological state.

Some major facts supporting the importance of MBL research in a clinical setting are:

• MBL deficiency
  • The most common congenital immunodeficiency occurring in 10% of the population ⁽¹⁾
  • A risk factor for infection in combination with an immunocompromised condition ⁽²⁾ 
  A risk factor for the occurrence of autoimmune diseases ⁽³⁾
  • Associated with increased susceptibility to sepsis ⁽⁴⁾ 
  
  
• Cardiovascular diseases
  • Prediction of decreased likelihood of myocardial infarction, particularly in diabetic patients ⁽⁵⁾
  • Serum levels indicate risk of future coronary artery disease in apparently healthy men but not in women ⁽⁶⁾
  • Serum levels are associated with vascular complications in type I diabetes ⁽⁷⁾
  
  
• Transplantation
  • MBL levels are associated with graft survival in kidney and heart transplantation ^(8,9)
  
  
• Cancer
  • MBL-deficiency is associated with severe infections after chemotherapy ⁽¹⁰⁾
  
  
• Apoptosis
  • MBL binds DNA fragments present in apoptotic cells initiating a cellular response ⁽¹¹⁾
  

Specifications

• The human MBL ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 4 hours
  
• After activation, samples and standards are captured by solid bound mannan
  
• Quantitative measurement of natural functional human MBL in serum and plasma
  
• Measurable concentration range of 0.41 – 100 ng/ml
  
• Sensitivity of 0.41 ng/ml
  
• Working volume 100 μl/well
  
• 40 samples in duplo
  
• Proprietary buffer prevents binding of C1q to immune complexes and disrupt the C1 complex, leaving the carbohydrate binding activity of MBL and the integrity of the MBL complex intact

Selected articles on MBL:

1. Thiel, S et al; Mol Immunol 2006, 43: 86
2. Boniotto, M et al; J Mol Med 2005, 83: 308
3. Tsutsumi, A et al; Autoimmun Rev 2005, 4:364
4. Gordon, A er al; Shock 2006, 25: 88
5. Saevarsdottir, S et al; J Exp Med 2005, 201: 117
6. Keller, T et al; Arterioscler Thromb Vasc Biol 2006, 26: 2345
7. Hansen, T et al; Diabetes 2004, 53:1570
8. Fiane, A et al; Eur Heart J 2005, 26: 1660
9. Berger, S et al; Am J Transplant 2005, 5: 1361
10. Mullighan, C et al ; Leuk Lymphoma 2004, 45:247
11. Palaniyar, N et al; J Biol Chem 2004, 279: 32728